Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense
NifA protein activates transcription of nitrogen fixation operons by the alternative sigma54 holoenzyme form of RNA polymerase.This protein binds to a well-defined upstream activator sequence (UAS) located at the -200/-100 position of nif promoters with the consensus motif TGT-N10-ACA.NifA of Azospirillum brasilense was purified in the form of a gl